Human brucellosis from consumption of unpasteurized milk in a rural area of Manabí

Authors

DOI:

https://doi.org/10.36097/rgcs.v3i1.3217

Keywords:

brucellosis, zoonosis, unpasteurized milk, rural areas, serological testing, Brucella diagnosis

Abstract

Brucellosis remains a significant zoonosis in various regions of the world and persists as a public health threat, especially in rural communities where the consumption of unpasteurized milk is still common. This practice increases exposure to infections caused by bacteria of the genus Brucella. The objective of this study was to analyze human brucellosis due to the consumption of unpasteurized milk in a rural area of Manabí. An observational, descriptive, and cross-sectional study was conducted among residents of the rural area of Olmedo. All participants underwent serological testing: agglutination of the febrile antigen for Brucella and an ELISA-based assay for the detection of IgG antibodies. One sample showed positive reactivity by agglutination, while all samples tested negative in the ELISA assay, indicating greater reliability and accuracy of the ELISA method for the diagnosis of Brucella. It was concluded that serological methods, such as the ELISA assay, should be used to prevent cases of brucellosis in rural areas.

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References

Chamorro, A. F. (2022). Sero-epidemiología e identificación de Brucella SPP. con técnicas moleculares en ganado bovino en la provincia del Carchi [Master's thesis, Universidad Internacional SEK]. https://repositorio.uisek.edu.ec/handle/123456789/4588

Garrido, A. D., Barrionuevo, M. Y., Moreno-Caballeros, P., Burbano-Enríquez, A., Salas, V. A., Galante, M. C., Wielick, C., Ron-Román, J., & Saegerman, C. (2025). Bayesian Estimation of the True Bovine Brucellosis Prevalence in Vaccinated and Non-Vaccinated Ecuadorian Cattle Populations, and the Sensitivity and Specificity of a Competitive and Indirect ELISA Using a New Synthetic Antigen. Microorganisms, 13(1), 69. https://doi.org/10.3390/microorganisms13010069

Garrido-Haro, A., Barrionuevo-Samaniego, M., Moreno-Caballeros, P., Burbano-Enríquez, A., Sánchez-Vázquez, MJ, Pompei, J., Humblet, M.-F., Ron-Román, J., & Saegerman, C. (2023). Seroprevalencia y factores de riesgo relacionados con la brucelosis bovina en el Ecuador continental. Pathogens, 12(9), 1134. https://doi.org/10.3390/pathogens12091134

Hurtado, J. A. (2017). La brucelosis: un problema de salud en la sombra. Enfermedades Infecciosas y Microbiología, 37(4), 115. https://www.medigraphic.com/cgi-bin/new/resumen.cgi?IDARTICULO=86243

Ibarra, E., López, E., López, F., & Silva, J. (2021). “Una sola salud”, (One Health): Estudio de caso brucelosis en Carchi – Ecuador. Horizontes de Enfermería, (11), 70-80. https://doi.org/10.32645/13906984.1085

Islam, M. S., Habib, M. A., Tonu, N. S., Haque, M. S., & Rahman, M. M. (2025). Beyond serology: A meta-analysis of advancements in molecular detection of Brucella spp. in seronegative animals and biological samples. Veterinary Medicine and Science, 11(1), e70200. https://doi.org/10.1002/vms3.70200

Loubet, P., Magnan, C., Salipante, F., Pastre, T., Keriel, A., O’Callaghan, D., Sotto, A., & Lavigne, J.-P. (2024). Diagnosis of brucellosis: Combining tests to improve performance. PLoS Neglected Tropical Diseases, 18(9), e0012442. https://doi.org/10.1371/journal.pntd.0012442

Lukambagire, A. S., Mendes, Â. J., Bodenham, R. F., McGiven, J. A., Mkenda, N. A., Mathew, C., Rubach, M. P., Sakasaka, P., Shayo, D. D., Maro, V. P., Shirima, G. M., Thomas, K. M., Kasanga, C. J., Kazwala, R. R., Halliday, J. E. B., & Mmbaga, B. T. (2021). Performance characteristics and costs of serological tests for brucellosis in a pastoralist community of northern Tanzania. Scientific Reports, 11(1), 5480. https://doi.org/10.1038/s41598-021-82906-w

Mondragón-Lenis, I. M., Vélez-Londoño, J. D., Call, D., Sánchez-Jiménez, M., & Cardona-Castro, N. (2020). Primer caso confirmado de brucelosis humana por Brucella melitensis, una zoonosis presente en Colombia. Infectio, 24(4), 259–261. https://doi.org/10.22354/in.v24i4.886

Ormazábal, M. C., Hidalgo, M., Salas, M., Benito, S. M., Pérez, A., & del Amo, C. (2022). Brucelosis humana. Revista Sanitaria de Investigación, 3(1). https://revistasanitariadeinvestigacion.com/brucelosis-humana/

Rivadeneira, P. N. (2022). Determinación de la prevalencia y factores de riesgo de brucelosis en explotaciones [Undergraduate thesis, Universidad de las Fuerzas Armadas]. https://repositoriobe.espe.edu.ec/server/api/core/bitstreams/28c48bd9-c601-47b8-be5c-2321b8287d92/content

Xu, N., Wang, W., Chen, F., Li, W., & Wang, G. (2020). ELISA is superior to bacterial culture and agglutination test in the diagnosis of brucellosis in an endemic area in China. BMC Infectious Diseases, 20(1), 11. https://doi.org/10.1186/s12879-019-4729-1

Published

2026-01-31

Data Availability Statement

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Issue

Section

Original articles

How to Cite

Mero, A. N., & Mieles, S. L. (2026). Human brucellosis from consumption of unpasteurized milk in a rural area of Manabí. Revista Gregoriana De Ciencias De La Salud, 3(1), 49-58. https://doi.org/10.36097/rgcs.v3i1.3217

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